Genomics of hybrid poplar (Populus trichocarpax deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences in poplar.
Identifieur interne : 003E06 ( Main/Exploration ); précédent : 003E05; suivant : 003E07Genomics of hybrid poplar (Populus trichocarpax deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences in poplar.
Auteurs : Steven Ralph [Canada] ; Claire Oddy ; Dawn Cooper ; Hesther Yueh ; Sharon Jancsik ; Natalia Kolosova ; Ryan N. Philippe ; Dana Aeschliman ; Rick White ; Dezene Huber ; Carol E. Ritland ; François Benoit ; Tracey Rigby ; André Nantel ; Yaron S N. Butterfield ; Robert Kirkpatrick ; Elizabeth Chun ; Jerry Liu ; Diana Palmquist ; Brian Wynhoven ; Jeffrey Stott ; George Yang ; Sarah Barber ; Robert A. Holt ; Asim Siddiqui ; Steven J M. Jones ; Marco A. Marra ; Brian E. Ellis ; Carl J. Douglas ; Kermit Ritland ; Jörg BohlmannSource :
- Molecular ecology [ 0962-1083 ] ; 2006.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Animaux (MeSH), Banque de gènes (MeSH), Enzymes (génétique), Génotype (MeSH), Hybridation d'acides nucléiques (MeSH), Lepidoptera (classification), Lepidoptera (génétique), Lepidoptera (pathogénicité), Maladies des plantes (microbiologie), Populus (génétique), Populus (microbiologie), Populus (métabolisme), Protéines d'insecte (génétique), Séquençage par oligonucléotides en batterie (MeSH), Transcription génétique (MeSH), Étiquettes de séquences exprimées (MeSH), Évolution moléculaire (MeSH).
- MESH :
- génétique : ADN complémentaire, Enzymes, Lepidoptera, Populus, Protéines d'insecte.
- microbiologie : Maladies des plantes, Populus.
- métabolisme : Populus.
- pathogénicité : Lepidoptera.
- Animaux, Banque de gènes, Génotype, Hybridation d'acides nucléiques, Séquençage par oligonucléotides en batterie, Transcription génétique, Étiquettes de séquences exprimées, Évolution moléculaire.
English descriptors
- KwdEn :
- Animals (MeSH), DNA, Complementary (genetics), Enzymes (genetics), Evolution, Molecular (MeSH), Expressed Sequence Tags (MeSH), Gene Library (MeSH), Genotype (MeSH), Insect Proteins (genetics), Lepidoptera (classification), Lepidoptera (genetics), Lepidoptera (pathogenicity), Nucleic Acid Hybridization (MeSH), Oligonucleotide Array Sequence Analysis (MeSH), Plant Diseases (microbiology), Populus (genetics), Populus (metabolism), Populus (microbiology), Transcription, Genetic (MeSH).
- MESH :
- chemical , genetics : DNA, Complementary, Enzymes, Insect Proteins.
- classification : Lepidoptera.
- genetics : Lepidoptera, Populus.
- metabolism : Populus.
- microbiology : Plant Diseases, Populus.
- pathogenicity : Lepidoptera.
- Animals, Evolution, Molecular, Expressed Sequence Tags, Gene Library, Genotype, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Transcription, Genetic.
Abstract
As part of a genomics strategy to characterize inducible defences against insect herbivory in poplar, we developed a comprehensive suite of functional genomics resources including cDNA libraries, expressed sequence tags (ESTs) and a cDNA microarray platform. These resources are designed to complement the existing poplar genome sequence and poplar (Populus spp.) ESTs by focusing on herbivore- and elicitor-treated tissues and incorporating normalization methods to capture rare transcripts. From a set of 15 standard, normalized or full-length cDNA libraries, we generated 139,007 3'- or 5'-end sequenced ESTs, representing more than one-third of the c. 385,000 publicly available Populus ESTs. Clustering and assembly of 107,519 3'-end ESTs resulted in 14,451 contigs and 20,560 singletons, altogether representing 35,011 putative unique transcripts, or potentially more than three-quarters of the predicted c. 45,000 genes in the poplar genome. Using this EST resource, we developed a cDNA microarray containing 15,496 unique genes, which was utilized to monitor gene expression in poplar leaves in response to herbivory by forest tent caterpillars (Malacosoma disstria). After 24 h of feeding, 1191 genes were classified as up-regulated, compared to only 537 down-regulated. Functional classification of this induced gene set revealed genes with roles in plant defence (e.g. endochitinases, Kunitz protease inhibitors), octadecanoid and ethylene signalling (e.g. lipoxygenase, allene oxide synthase, 1-aminocyclopropane-1-carboxylate oxidase), transport (e.g. ABC proteins, calreticulin), secondary metabolism [e.g. polyphenol oxidase, isoflavone reductase, (-)-germacrene D synthase] and transcriptional regulation [e.g. leucine-rich repeat transmembrane kinase, several transcription factor classes (zinc finger C3H type, AP2/EREBP, WRKY, bHLH)]. This study provides the first genome-scale approach to characterize insect-induced defences in a woody perennial providing a solid platform for functional investigation of plant-insect interactions in poplar.
DOI: 10.1111/j.1365-294X.2006.02824.x
PubMed: 16626454
Affiliations:
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Douglas</name></author><author><name sortKey="Ritland, Kermit" sort="Ritland, Kermit" uniqKey="Ritland K" first="Kermit" last="Ritland">Kermit Ritland</name></author><author><name sortKey="Bohlmann, Jorg" sort="Bohlmann, Jorg" uniqKey="Bohlmann J" first="Jörg" last="Bohlmann">Jörg Bohlmann</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16626454</idno><idno type="pmid">16626454</idno><idno type="doi">10.1111/j.1365-294X.2006.02824.x</idno><idno type="wicri:Area/Main/Corpus">003E25</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003E25</idno><idno type="wicri:Area/Main/Curation">003E25</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003E25</idno><idno type="wicri:Area/Main/Exploration">003E25</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genomics of hybrid poplar (Populus trichocarpax deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences in poplar.</title><author><name sortKey="Ralph, Steven" sort="Ralph, Steven" uniqKey="Ralph S" first="Steven" last="Ralph">Steven Ralph</name><affiliation wicri:level="4"><nlm:affiliation>Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3.</nlm:affiliation><orgName type="university">Université de la Colombie-Britannique</orgName><country>Canada</country><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Oddy, Claire" sort="Oddy, Claire" uniqKey="Oddy C" first="Claire" last="Oddy">Claire Oddy</name></author><author><name sortKey="Cooper, Dawn" sort="Cooper, Dawn" uniqKey="Cooper D" first="Dawn" last="Cooper">Dawn Cooper</name></author><author><name sortKey="Yueh, Hesther" sort="Yueh, Hesther" uniqKey="Yueh H" first="Hesther" last="Yueh">Hesther Yueh</name></author><author><name sortKey="Jancsik, Sharon" sort="Jancsik, Sharon" uniqKey="Jancsik S" first="Sharon" last="Jancsik">Sharon Jancsik</name></author><author><name sortKey="Kolosova, Natalia" sort="Kolosova, Natalia" uniqKey="Kolosova N" first="Natalia" last="Kolosova">Natalia Kolosova</name></author><author><name sortKey="Philippe, Ryan N" sort="Philippe, Ryan N" uniqKey="Philippe R" first="Ryan N" last="Philippe">Ryan N. Philippe</name></author><author><name sortKey="Aeschliman, Dana" sort="Aeschliman, Dana" uniqKey="Aeschliman D" first="Dana" last="Aeschliman">Dana Aeschliman</name></author><author><name sortKey="White, Rick" sort="White, Rick" uniqKey="White R" first="Rick" last="White">Rick White</name></author><author><name sortKey="Huber, Dezene" sort="Huber, Dezene" uniqKey="Huber D" first="Dezene" last="Huber">Dezene Huber</name></author><author><name sortKey="Ritland, Carol E" sort="Ritland, Carol E" uniqKey="Ritland C" first="Carol E" last="Ritland">Carol E. Ritland</name></author><author><name sortKey="Benoit, Francois" sort="Benoit, Francois" uniqKey="Benoit F" first="François" last="Benoit">François Benoit</name></author><author><name sortKey="Rigby, Tracey" sort="Rigby, Tracey" uniqKey="Rigby T" first="Tracey" last="Rigby">Tracey Rigby</name></author><author><name sortKey="Nantel, Andre" sort="Nantel, Andre" uniqKey="Nantel A" first="André" last="Nantel">André Nantel</name></author><author><name sortKey="Butterfield, Yaron S N" sort="Butterfield, Yaron S N" uniqKey="Butterfield Y" first="Yaron S N" last="Butterfield">Yaron S N. Butterfield</name></author><author><name sortKey="Kirkpatrick, Robert" sort="Kirkpatrick, Robert" uniqKey="Kirkpatrick R" first="Robert" last="Kirkpatrick">Robert Kirkpatrick</name></author><author><name sortKey="Chun, Elizabeth" sort="Chun, Elizabeth" uniqKey="Chun E" first="Elizabeth" last="Chun">Elizabeth Chun</name></author><author><name sortKey="Liu, Jerry" sort="Liu, Jerry" uniqKey="Liu J" first="Jerry" last="Liu">Jerry Liu</name></author><author><name sortKey="Palmquist, Diana" sort="Palmquist, Diana" uniqKey="Palmquist D" first="Diana" last="Palmquist">Diana Palmquist</name></author><author><name sortKey="Wynhoven, Brian" sort="Wynhoven, Brian" uniqKey="Wynhoven B" first="Brian" last="Wynhoven">Brian Wynhoven</name></author><author><name sortKey="Stott, Jeffrey" sort="Stott, Jeffrey" uniqKey="Stott J" first="Jeffrey" last="Stott">Jeffrey Stott</name></author><author><name sortKey="Yang, George" sort="Yang, George" uniqKey="Yang G" first="George" last="Yang">George Yang</name></author><author><name sortKey="Barber, Sarah" sort="Barber, Sarah" uniqKey="Barber S" first="Sarah" last="Barber">Sarah Barber</name></author><author><name sortKey="Holt, Robert A" sort="Holt, Robert A" uniqKey="Holt R" first="Robert A" last="Holt">Robert A. Holt</name></author><author><name sortKey="Siddiqui, Asim" sort="Siddiqui, Asim" uniqKey="Siddiqui A" first="Asim" last="Siddiqui">Asim Siddiqui</name></author><author><name sortKey="Jones, Steven J M" sort="Jones, Steven J M" uniqKey="Jones S" first="Steven J M" last="Jones">Steven J M. Jones</name></author><author><name sortKey="Marra, Marco A" sort="Marra, Marco A" uniqKey="Marra M" first="Marco A" last="Marra">Marco A. Marra</name></author><author><name sortKey="Ellis, Brian E" sort="Ellis, Brian E" uniqKey="Ellis B" first="Brian E" last="Ellis">Brian E. Ellis</name></author><author><name sortKey="Douglas, Carl J" sort="Douglas, Carl J" uniqKey="Douglas C" first="Carl J" last="Douglas">Carl J. Douglas</name></author><author><name sortKey="Ritland, Kermit" sort="Ritland, Kermit" uniqKey="Ritland K" first="Kermit" last="Ritland">Kermit Ritland</name></author><author><name sortKey="Bohlmann, Jorg" sort="Bohlmann, Jorg" uniqKey="Bohlmann J" first="Jörg" last="Bohlmann">Jörg Bohlmann</name></author></analytic><series><title level="j">Molecular ecology</title><idno type="ISSN">0962-1083</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>DNA, Complementary (genetics)</term><term>Enzymes (genetics)</term><term>Evolution, Molecular (MeSH)</term><term>Expressed Sequence Tags (MeSH)</term><term>Gene Library (MeSH)</term><term>Genotype (MeSH)</term><term>Insect Proteins (genetics)</term><term>Lepidoptera (classification)</term><term>Lepidoptera (genetics)</term><term>Lepidoptera (pathogenicity)</term><term>Nucleic Acid Hybridization (MeSH)</term><term>Oligonucleotide Array Sequence Analysis (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Populus (microbiology)</term><term>Transcription, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>Animaux (MeSH)</term><term>Banque de gènes (MeSH)</term><term>Enzymes (génétique)</term><term>Génotype (MeSH)</term><term>Hybridation d'acides nucléiques (MeSH)</term><term>Lepidoptera (classification)</term><term>Lepidoptera (génétique)</term><term>Lepidoptera (pathogénicité)</term><term>Maladies des plantes (microbiologie)</term><term>Populus (génétique)</term><term>Populus (microbiologie)</term><term>Populus (métabolisme)</term><term>Protéines d'insecte (génétique)</term><term>Séquençage par oligonucléotides en batterie (MeSH)</term><term>Transcription génétique (MeSH)</term><term>Étiquettes de séquences exprimées (MeSH)</term><term>Évolution moléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Enzymes</term><term>Insect Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Lepidoptera</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lepidoptera</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Enzymes</term><term>Lepidoptera</term><term>Populus</term><term>Protéines d'insecte</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Lepidoptera</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Lepidoptera</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Evolution, Molecular</term><term>Expressed Sequence Tags</term><term>Gene Library</term><term>Genotype</term><term>Nucleic Acid Hybridization</term><term>Oligonucleotide Array Sequence Analysis</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Banque de gènes</term><term>Génotype</term><term>Hybridation d'acides nucléiques</term><term>Séquençage par oligonucléotides en batterie</term><term>Transcription génétique</term><term>Étiquettes de séquences exprimées</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">As part of a genomics strategy to characterize inducible defences against insect herbivory in poplar, we developed a comprehensive suite of functional genomics resources including cDNA libraries, expressed sequence tags (ESTs) and a cDNA microarray platform. These resources are designed to complement the existing poplar genome sequence and poplar (Populus spp.) ESTs by focusing on herbivore- and elicitor-treated tissues and incorporating normalization methods to capture rare transcripts. From a set of 15 standard, normalized or full-length cDNA libraries, we generated 139,007 3'- or 5'-end sequenced ESTs, representing more than one-third of the c. 385,000 publicly available Populus ESTs. Clustering and assembly of 107,519 3'-end ESTs resulted in 14,451 contigs and 20,560 singletons, altogether representing 35,011 putative unique transcripts, or potentially more than three-quarters of the predicted c. 45,000 genes in the poplar genome. Using this EST resource, we developed a cDNA microarray containing 15,496 unique genes, which was utilized to monitor gene expression in poplar leaves in response to herbivory by forest tent caterpillars (Malacosoma disstria). After 24 h of feeding, 1191 genes were classified as up-regulated, compared to only 537 down-regulated. Functional classification of this induced gene set revealed genes with roles in plant defence (e.g. endochitinases, Kunitz protease inhibitors), octadecanoid and ethylene signalling (e.g. lipoxygenase, allene oxide synthase, 1-aminocyclopropane-1-carboxylate oxidase), transport (e.g. ABC proteins, calreticulin), secondary metabolism [e.g. polyphenol oxidase, isoflavone reductase, (-)-germacrene D synthase] and transcriptional regulation [e.g. leucine-rich repeat transmembrane kinase, several transcription factor classes (zinc finger C3H type, AP2/EREBP, WRKY, bHLH)]. This study provides the first genome-scale approach to characterize insect-induced defences in a woody perennial providing a solid platform for functional investigation of plant-insect interactions in poplar.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16626454</PMID><DateCompleted><Year>2006</Year><Month>06</Month><Day>23</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0962-1083</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><Issue>5</Issue><PubDate><Year>2006</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Molecular ecology</Title><ISOAbbreviation>Mol Ecol</ISOAbbreviation></Journal><ArticleTitle>Genomics of hybrid poplar (Populus trichocarpax deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences in poplar.</ArticleTitle><Pagination><MedlinePgn>1275-97</MedlinePgn></Pagination><Abstract><AbstractText>As part of a genomics strategy to characterize inducible defences against insect herbivory in poplar, we developed a comprehensive suite of functional genomics resources including cDNA libraries, expressed sequence tags (ESTs) and a cDNA microarray platform. These resources are designed to complement the existing poplar genome sequence and poplar (Populus spp.) ESTs by focusing on herbivore- and elicitor-treated tissues and incorporating normalization methods to capture rare transcripts. From a set of 15 standard, normalized or full-length cDNA libraries, we generated 139,007 3'- or 5'-end sequenced ESTs, representing more than one-third of the c. 385,000 publicly available Populus ESTs. Clustering and assembly of 107,519 3'-end ESTs resulted in 14,451 contigs and 20,560 singletons, altogether representing 35,011 putative unique transcripts, or potentially more than three-quarters of the predicted c. 45,000 genes in the poplar genome. Using this EST resource, we developed a cDNA microarray containing 15,496 unique genes, which was utilized to monitor gene expression in poplar leaves in response to herbivory by forest tent caterpillars (Malacosoma disstria). After 24 h of feeding, 1191 genes were classified as up-regulated, compared to only 537 down-regulated. Functional classification of this induced gene set revealed genes with roles in plant defence (e.g. endochitinases, Kunitz protease inhibitors), octadecanoid and ethylene signalling (e.g. lipoxygenase, allene oxide synthase, 1-aminocyclopropane-1-carboxylate oxidase), transport (e.g. ABC proteins, calreticulin), secondary metabolism [e.g. polyphenol oxidase, isoflavone reductase, (-)-germacrene D synthase] and transcriptional regulation [e.g. leucine-rich repeat transmembrane kinase, several transcription factor classes (zinc finger C3H type, AP2/EREBP, WRKY, bHLH)]. This study provides the first genome-scale approach to characterize insect-induced defences in a woody perennial providing a solid platform for functional investigation of plant-insect interactions in poplar.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>Steven</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oddy</LastName><ForeName>Claire</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Cooper</LastName><ForeName>Dawn</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Yueh</LastName><ForeName>Hesther</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Jancsik</LastName><ForeName>Sharon</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Kolosova</LastName><ForeName>Natalia</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Philippe</LastName><ForeName>Ryan N</ForeName><Initials>RN</Initials></Author><Author ValidYN="Y"><LastName>Aeschliman</LastName><ForeName>Dana</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>White</LastName><ForeName>Rick</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Huber</LastName><ForeName>Dezene</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Ritland</LastName><ForeName>Carol E</ForeName><Initials>CE</Initials></Author><Author ValidYN="Y"><LastName>Benoit</LastName><ForeName>François</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Rigby</LastName><ForeName>Tracey</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Nantel</LastName><ForeName>André</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Butterfield</LastName><ForeName>Yaron S N</ForeName><Initials>YS</Initials></Author><Author ValidYN="Y"><LastName>Kirkpatrick</LastName><ForeName>Robert</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Chun</LastName><ForeName>Elizabeth</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Jerry</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Palmquist</LastName><ForeName>Diana</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Wynhoven</LastName><ForeName>Brian</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Stott</LastName><ForeName>Jeffrey</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>George</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Barber</LastName><ForeName>Sarah</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Holt</LastName><ForeName>Robert A</ForeName><Initials>RA</Initials></Author><Author ValidYN="Y"><LastName>Siddiqui</LastName><ForeName>Asim</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Jones</LastName><ForeName>Steven J M</ForeName><Initials>SJ</Initials></Author><Author ValidYN="Y"><LastName>Marra</LastName><ForeName>Marco A</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Ellis</LastName><ForeName>Brian E</ForeName><Initials>BE</Initials></Author><Author ValidYN="Y"><LastName>Douglas</LastName><ForeName>Carl J</ForeName><Initials>CJ</Initials></Author><Author ValidYN="Y"><LastName>Ritland</LastName><ForeName>Kermit</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Bohlmann</LastName><ForeName>Jörg</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Mol Ecol</MedlineTA><NlmUniqueID>9214478</NlmUniqueID><ISSNLinking>0962-1083</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004798">Enzymes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019476">Insect Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004798" MajorTopicYN="N">Enzymes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="N">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020224" MajorTopicYN="N">Expressed Sequence Tags</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019476" MajorTopicYN="N">Insect Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007915" MajorTopicYN="N">Lepidoptera</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009693" MajorTopicYN="N">Nucleic Acid Hybridization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>4</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>6</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>4</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16626454</ArticleId><ArticleId IdType="pii">MEC2824</ArticleId><ArticleId IdType="doi">10.1111/j.1365-294X.2006.02824.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Aeschliman, Dana" sort="Aeschliman, Dana" uniqKey="Aeschliman D" first="Dana" last="Aeschliman">Dana Aeschliman</name><name sortKey="Barber, Sarah" sort="Barber, Sarah" uniqKey="Barber S" first="Sarah" last="Barber">Sarah Barber</name><name sortKey="Benoit, Francois" sort="Benoit, Francois" uniqKey="Benoit F" first="François" last="Benoit">François Benoit</name><name sortKey="Bohlmann, Jorg" sort="Bohlmann, Jorg" uniqKey="Bohlmann J" first="Jörg" last="Bohlmann">Jörg Bohlmann</name><name sortKey="Butterfield, Yaron S N" sort="Butterfield, Yaron S N" uniqKey="Butterfield Y" first="Yaron S N" last="Butterfield">Yaron S N. Butterfield</name><name sortKey="Chun, Elizabeth" sort="Chun, Elizabeth" uniqKey="Chun E" first="Elizabeth" last="Chun">Elizabeth Chun</name><name sortKey="Cooper, Dawn" sort="Cooper, Dawn" uniqKey="Cooper D" first="Dawn" last="Cooper">Dawn Cooper</name><name sortKey="Douglas, Carl J" sort="Douglas, Carl J" uniqKey="Douglas C" first="Carl J" last="Douglas">Carl J. Douglas</name><name sortKey="Ellis, Brian E" sort="Ellis, Brian E" uniqKey="Ellis B" first="Brian E" last="Ellis">Brian E. Ellis</name><name sortKey="Holt, Robert A" sort="Holt, Robert A" uniqKey="Holt R" first="Robert A" last="Holt">Robert A. Holt</name><name sortKey="Huber, Dezene" sort="Huber, Dezene" uniqKey="Huber D" first="Dezene" last="Huber">Dezene Huber</name><name sortKey="Jancsik, Sharon" sort="Jancsik, Sharon" uniqKey="Jancsik S" first="Sharon" last="Jancsik">Sharon Jancsik</name><name sortKey="Jones, Steven J M" sort="Jones, Steven J M" uniqKey="Jones S" first="Steven J M" last="Jones">Steven J M. Jones</name><name sortKey="Kirkpatrick, Robert" sort="Kirkpatrick, Robert" uniqKey="Kirkpatrick R" first="Robert" last="Kirkpatrick">Robert Kirkpatrick</name><name sortKey="Kolosova, Natalia" sort="Kolosova, Natalia" uniqKey="Kolosova N" first="Natalia" last="Kolosova">Natalia Kolosova</name><name sortKey="Liu, Jerry" sort="Liu, Jerry" uniqKey="Liu J" first="Jerry" last="Liu">Jerry Liu</name><name sortKey="Marra, Marco A" sort="Marra, Marco A" uniqKey="Marra M" first="Marco A" last="Marra">Marco A. Marra</name><name sortKey="Nantel, Andre" sort="Nantel, Andre" uniqKey="Nantel A" first="André" last="Nantel">André Nantel</name><name sortKey="Oddy, Claire" sort="Oddy, Claire" uniqKey="Oddy C" first="Claire" last="Oddy">Claire Oddy</name><name sortKey="Palmquist, Diana" sort="Palmquist, Diana" uniqKey="Palmquist D" first="Diana" last="Palmquist">Diana Palmquist</name><name sortKey="Philippe, Ryan N" sort="Philippe, Ryan N" uniqKey="Philippe R" first="Ryan N" last="Philippe">Ryan N. Philippe</name><name sortKey="Rigby, Tracey" sort="Rigby, Tracey" uniqKey="Rigby T" first="Tracey" last="Rigby">Tracey Rigby</name><name sortKey="Ritland, Carol E" sort="Ritland, Carol E" uniqKey="Ritland C" first="Carol E" last="Ritland">Carol E. Ritland</name><name sortKey="Ritland, Kermit" sort="Ritland, Kermit" uniqKey="Ritland K" first="Kermit" last="Ritland">Kermit Ritland</name><name sortKey="Siddiqui, Asim" sort="Siddiqui, Asim" uniqKey="Siddiqui A" first="Asim" last="Siddiqui">Asim Siddiqui</name><name sortKey="Stott, Jeffrey" sort="Stott, Jeffrey" uniqKey="Stott J" first="Jeffrey" last="Stott">Jeffrey Stott</name><name sortKey="White, Rick" sort="White, Rick" uniqKey="White R" first="Rick" last="White">Rick White</name><name sortKey="Wynhoven, Brian" sort="Wynhoven, Brian" uniqKey="Wynhoven B" first="Brian" last="Wynhoven">Brian Wynhoven</name><name sortKey="Yang, George" sort="Yang, George" uniqKey="Yang G" first="George" last="Yang">George Yang</name><name sortKey="Yueh, Hesther" sort="Yueh, Hesther" uniqKey="Yueh H" first="Hesther" last="Yueh">Hesther Yueh</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Ralph, Steven" sort="Ralph, Steven" uniqKey="Ralph S" first="Steven" last="Ralph">Steven Ralph</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Genomics of hybrid poplar (Populus trichocarpax deltoides) interacting with forest tent caterpillars (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect-induced defences in poplar.
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